ABSTRACT
Content marketing is becoming an important trend in the tourism industry and is attracting the attention of many stakeholders. Previous studies have sporadically highlighted only some content marketing aspects, but none comprehensively described the topic. To fill this gap, this study adopts a comprehensive approach by combining two bibliometric co-citation and co-keyword analysis methods of 659 articles on content marketing in travel sectors. The co-citation results indicate that tourism content marketing research has been concentrated on the themes of (1) the impact of electronic word of mouth (eWOM) and word of mouth (WOM) on business performance; (2) the role of social media, user-generated content (UGC) and destination image formulation; (3) the impact of eWOM and UGC on the decision-making process; as well as (4) opportunities and challenges. Additionally, based on the co-keyword analysis, hot research topics are explored, including online review implementation; UGC implementation; communication and information search; customer behavior prediction model; the decision-making process; and issues related to user experience, quality, and management. Among these, UGC implementation is the most likely trend that researchers can develop in the future. In addition, the influence of other types of UGC (e.g., user-generated travel videos) is a promising avenue for future research. This study will help researchers understand the role and influence of tourism content marketing. Furthermore, tourism marketers can use content marketing to restore destination image and address last-minute booking issues after the COVID-19 pandemic.
ABSTRACT
BackgroundThe COVID‐19 pandemic caused by SARS‐CoV‐2 remains public health burdens and many unresolved issues worldwide. Molecular assays based on real‐time RT‐PCR are critical for the detection of SARS‐CoV‐2 in clinical specimens from patients suspected of COVID‐19.ObjectiveWe aimed to establish and validate an in‐house real‐time RT‐PCR for the detection of SARS‐CoV‐2.MethodologyPrimers and probes sets in our in‐house real‐time RT‐PCR assay were designed in conserved regions of the N and E target genes. Optimized multiplex real‐time RT‐PCR assay was validated using the first WHO International Standard (NIBSC code: 20/146) and evaluated clinical performance.ResultsThe limit of detection validated using the first WHO International Standard was 159 IU/ml for both E and N target genes. The evaluation of clinical performance on 170 clinical samples showed a positive percent agreement of 100% and the negative percent agreement of 99.08% for both target genes. The Kappa value of 0.99 was an excellent agreement, the strong correlation of Ct values observed between two tests with r2 = 0.84 for the E gene and 0.87 for the N gene. Notably, we assessed on 60 paired saliva and nasopharyngeal samples. The overall agreement was 91.66%, and Kappa value of 0.74 showed a high agreement between two types of samples. When using nasopharyngeal swabs as the reference standard, positive percent agreement, and negative percent agreement were 91.83% and 90.90%, respectively.ConclusionIn the present study, we established and validated an in‐house real‐time RT‐PCR for molecular detection of SARS‐CoV‐2 in a resource‐limited country.